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solid pin multi blot replicators  (V&P Scientific)


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    V&P Scientific solid pin multi blot replicators
    Solid Pin Multi Blot Replicators, supplied by V&P Scientific, used in various techniques. Bioz Stars score: 94/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/solid+pin+multi+blot+replicators/VP+409/us12403104-650-24-28
    Average 94 stars, based on 112 article reviews
    solid pin multi blot replicators - by Bioz Stars, 2026-09
    94/100 stars

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    Concentration Assay:

    Article Title: Compounds and methods of promoting myelination
    Article Snippet: .. For dose-response testing of all molecules except sterols, a 1000× compound stock in dimethyl sulphoxide (DMSO) was added to assay plates with 0.1 μL solid pin multi-blot replicators (V & P Scientific; VP 409), resulting in a final primary screening concentration of 1× Sterols were added to cells as an ethanol solution (0.2% final ethanol concentration). ..

    Article Title: Accumulation of 8,9-unsaturated sterols drives oligodendrocyte formation and remyelination
    Article Snippet: .. For dose–response testing of all molecules except sterols, a 1000x compound stock in dimethyl sulphoxide (DMSO) was added to assay plates with 0.1 μL solid pin multi-blot replicators (V & P Scientific; VP 409), resulting in a final primary screening concentration of 1x. ..

    Article Title: Diverse chemical scaffolds enhance oligodendrocyte formation by inhibiting CYP51, TM7SF2, or EBP
    Article Snippet: .. For dose–response testing of all molecules, a 1000× compound stock in dimethyl sulphoxide (DMSO) was added to assay plates with 0.1 μL solid pin multi-blot replicators (V & P Scientific; VP 409), resulting in a final screening concentration of 1×. ..

    Article Title: Compounds and methods of promoting myelination
    Article Snippet: .. A 1000× compound stock in dimethyl sulphoxide (DMSO) was added to assay plates with 0.1 mL solid pin multi-blot replicators (V & P Scientific; VP 409), resulting in a final primary screening concentration of 1×. ..

    Article Title: Compounds for treating myelin related disorders
    Article Snippet: .. For dose-response testing of all molecules except sterols, a 1000× compound stock in dimethyl sulphoxide (DMSO) was added to assay plates with 0.1 μL solid pin multi-blot replicators (V & P Scientific; VP 409), resulting in a final primary screening concentration of 1×. ..

    Article Title: Compounds and methods of promoting myelination
    Article Snippet: .. For dose-response testing of all molecules except sterols, a 1000× compound stock in dimethyl sulphoxide (DMSO) was added to assay plates with 0.1 μL solid pin multi-blot replicators (V & P Scientific; VP 409), resulting in a final primary screening concentration of 1×. ..



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    solid pin multi blot replicators  (V&P Scientific)
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    Construction of the <t>384-format</t> mutant array guided by the library copier VP381 Yeast deletion library colonies grown on the source plates (YPD agar plate containing G418) were created by inoculation from frozen glycerol stock in 96-format arrays. Before the subsequent pinning steps, fit the destination plate (YPD agar plate containing G418) into the middle of the library copier VP381. To condense four 96-format arrays into a single 384-format array, pin the colonies on the first source plate using a sterile 96-pin <t>replicator</t> and replicate onto the destination plate aligned to the ‘A’ alignment holes. Repeat the pinning step with the next three source plates aligned to the ‘B’, ‘C’, and ‘D’ alignment holes and transfer to the same destination plate. The resulting 384-format mutant array will then be used for subsequent SGA steps to generate the final mutant array expressing the UPR sensors and reporters. Each 384-format array can be expanded to four 96-format liquid culture for flow cytometry acquisition.
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    Construction of the 384-format mutant array guided by the library copier VP381 Yeast deletion library colonies grown on the source plates (YPD agar plate containing G418) were created by inoculation from frozen glycerol stock in 96-format arrays. Before the subsequent pinning steps, fit the destination plate (YPD agar plate containing G418) into the middle of the library copier VP381. To condense four 96-format arrays into a single 384-format array, pin the colonies on the first source plate using a sterile 96-pin replicator and replicate onto the destination plate aligned to the ‘A’ alignment holes. Repeat the pinning step with the next three source plates aligned to the ‘B’, ‘C’, and ‘D’ alignment holes and transfer to the same destination plate. The resulting 384-format mutant array will then be used for subsequent SGA steps to generate the final mutant array expressing the UPR sensors and reporters. Each 384-format array can be expanded to four 96-format liquid culture for flow cytometry acquisition.

    Journal: STAR Protocols

    Article Title: A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiae

    doi: 10.1016/j.xpro.2021.100868

    Figure Lengend Snippet: Construction of the 384-format mutant array guided by the library copier VP381 Yeast deletion library colonies grown on the source plates (YPD agar plate containing G418) were created by inoculation from frozen glycerol stock in 96-format arrays. Before the subsequent pinning steps, fit the destination plate (YPD agar plate containing G418) into the middle of the library copier VP381. To condense four 96-format arrays into a single 384-format array, pin the colonies on the first source plate using a sterile 96-pin replicator and replicate onto the destination plate aligned to the ‘A’ alignment holes. Repeat the pinning step with the next three source plates aligned to the ‘B’, ‘C’, and ‘D’ alignment holes and transfer to the same destination plate. The resulting 384-format mutant array will then be used for subsequent SGA steps to generate the final mutant array expressing the UPR sensors and reporters. Each 384-format array can be expanded to four 96-format liquid culture for flow cytometry acquisition.

    Article Snippet: 384 Solid Pin Multi-Blot Replicator , V&P Scientific , VP 384F.

    Techniques: Mutagenesis, Sterility, Expressing, Flow Cytometry

    Journal: STAR Protocols

    Article Title: A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiae

    doi: 10.1016/j.xpro.2021.100868

    Figure Lengend Snippet:

    Article Snippet: 384 Solid Pin Multi-Blot Replicator , V&P Scientific , VP 384F.

    Techniques: Recombinant, Clone Assay, Software, Sterility, Microscopy